雷公藤T_4单体对体外培养的肾小球系膜细胞增殖及白介素－1产生的影响

Inhibitory Effects of Monomer T4 from Tripterygium Wilfordii Hook on Cultured Mesangial Cells Proliferation and IL-1 Production

无菌条件下分离提取人胚胎肾小球体外培养，以传代３～５次的系膜细胞，采用３Ｈ－胸腺嘧啶核苷（￣３Ｈ－ＴｄＲ）掺入法和胸腺细胞增殖法观察在不同浓度和不同作用时间雷公藤单体Ｔ_４对系膜细胞增殖及其白介素－１（ＩＬ－１）产生的影响。结果显示，雷公藤单体Ｔ_４明显抑制系膜细胞增殖及ＩＬ－１产生，其抑制作用的强弱呈剂量和时间依赖关系。本实验为进一步探讨雷公藤的免疫抑制作用和治疗肾小球疾病的机理提供了新的、有意义的实验依据。

The cultured mesangial cell(MSc)went through three serial passages before being us ing.Quantitation of MSc growth rate was obtained by measuring the ratio of 3H+TdR uptake in a medium containg various concertrations of T4.IL-1 activity was tested by 3 H-TdR in corporation in C578L/c mice thymocytes.The results show that T4 inhibited MSc prolifera tion and IL-1 production.