G－CSF酶联免疫检测试剂盒在发热性疾病鉴别诊断中的应用

Preparation and Application of ELISA Kit for Detection of G-CSF

用两种抗粒细胞集落刺激因子（Ｇ－ＣＳＦ）单克隆抗体，本院自制了Ｇ－ＣＳＦ酶联免疫检测试剂盒。经国内７家大医院试用，对临床标本测定的结果表明：细菌培养阳性发热患者（ｎ＝３１），Ｇ－ＣＳＦ阳性检出率为１００％。未做细菌培养临床诊断为细菌感染发热患者（ｎ＝２６９），Ｇ－ＣＳＦ阳性率为８９．２％；非细菌感染发热患者（ｎ＝１５３），Ｇ－ＣＳＦ阳性率只有６．５％；正常人（ｎ＝１５７），Ｇ－ＣＳＦ检测全部为阴性。证实本方法可用于发热性疾病的诊断、鉴别诊断，并可指导临床抗生素的合理使用。

By adding monoclonal antibodies from BALB/c mice to the granulocyte colony-stimulating factor(G-CSF),we established a sandwich ELISA for detecting G-CSF levels in human serum samples. G-CSF was measured in 157 normal serum samples and in 153 serum samples from patients with no clinical manifestation of bacterial infection and in 269 serum samples from patients with clinical diagnosis of bacterial infection and in 31 serum samples from patients with positive bacterial infection according to cell culture.It was found that the positive rates of G- CSF were zero in normal serum samples, only 6.5%in patients with no clinical manifestation of bacterial infection, 89.2%in patients with clinical diagnosis of bacterial infection and 100%in patients with bacterial culture positive for infection. Our results reveal that the sandwich ELISA for detection of G-CSF levels in human serum samples may be useful for diagnosing patients with bacterial infection and for clinically guiding the rational use of antibiotics.