补肾活血煎有效成分提取的工艺研究

Studies on Extracting Factors of Bushen Huoxue Jian

采用正交试验法,以淫羊藿苷含量、总黄酮含量、醇浸出物量(φ乙醇=0.8)为考核指标进行综合评价,比较水提和醇提工艺,优选补肾活血煎有效成分的提取工艺条件。结果表明:φ=0.5乙醇提取效果较水提好。最佳提取工艺条件为用8倍量φ=0.5乙醇回流提取3次,第一次1.5h,第二、三次各1h。利用最佳工艺条件能充分有效地提取药材中的有效成分。

Orthogonal design was used to study the optimum extracting factors of bushen huoxue jian. Results of water-extracting and ψ= 0.5 alcohol-extracing, taking the content of icariin, total flavonoid and φ=0.8 alcohol extracted substance as markers, were compared, which showed that the effect of φ=0.5 alcohol-extracting was better than that of water-extracting. The preparation procedure is as follows: extracted by φ=0.5 alcohol, with reflux 3 times for 1. 5 h, 1 h, 1 h, the amount of φ=0.5 alcohol was 8 times as much as the medicine material. According to the optimum extracting factors, the effective substance can be extracted efficiently. The extraction process optimized is stable and easy to operate, and suitable suit to industrialized production.