摘要
Ribosomal protein S 13 gene has been cloned and analyzed in many organisms,but there are few documents relating to insects. In this communication, the full-length cDNA sequence of ribosomal protein S 13 gene in the diamondback moth, Plutella xylostella(Lepidoptera: Plutellidae), was determined by using PCR amplification technique. The features of the ribosomal protein S 13 gene sequence were analyzed and the deduced amino acids sequence was compared with those from other insects. The results of multi-alignment of the amino acid sequences between the diamondback moth and other insect species revealed that this gene sequence is highly conserved in insects. Based on maximum likelihood method, a phylogenetic tree was constructed from 10 different species using PHYLIP software. It showed that nematode is one separate lineage and the five insect speciesbe long to another lineage, whereas those species higher than insects form the third one. The pattern of this phylogenetic tree evidently represented the evolution of different species.
Ribosomal protein S13 gene has been cloned and analyzed in many organisms,but there are few documents relating to insects. In this communication, the full-length cDNAsequence of ribosomal protein S13 gene in the diamondback moth, Plutella xylostella (Lepidoptera:Plutellidae), was determined by using PCR amplification technique. The features of the ribosomalprotein S13 gene sequence were analyzed and the deduced amino acids sequence was compared with thosefrom other insects. The results of multi-alignment of the amino acid sequences between thediamondback moth and other insect species revealed that this gene sequence is highly conserved ininsects. Based on maximum likelihood method, a phylogenetic tree was constructed from 10 differentspecies using PHYLIP software. It showed that nematode is one separate lineage and the five insectspecies belong to another lineage, whereas those species higher than insects form the third one. Thepattern of this phylogenetic tree evidently represented the evolution of different species.
