BTV及其dsRNA分子诱导IFN产生和细胞凋亡

BTV and its dsRNA Inducing IFN Production and Cell Apoptosis

在离体培养细胞上探讨BTV(BluetongueVirus,BTV)和BTVdsRNA分子诱导人宫颈癌Hela细胞IFN(Interferon)产生和凋亡的生物医学作用。离体培养的人宫颈癌Hela细胞单层分别施以不同水平的蓝舌病毒(BTV)和脂质体包裹的BTVdsRNA等因素处理,比较研究不同处理后细胞形态学差异;并通过MTT法检测不同处理条件下细胞存活率的差异;ELISA试剂盒检测培养细胞上清液中IFN含量及流式细胞仪检测细胞凋亡率。分析了不同处理条件下不同用量的病毒及不同浓度的RNA脂质体复合物诱导培养细胞产生IFN量的差异,揭示了不同因素和不同水平处理下,Hela细胞IFN产量具有显著性差异(p<0.05),而BTVdsRNA诱导人宫颈癌Hela细胞凋亡能力低于BTV(p<0.05)。BTVdsRNA可显著诱导人宫颈癌细胞产生IFN和凋亡,推断BTVdsRNA具有发展成为新的干扰素诱导剂的潜能。

To study the biological medicine function between BTV and BTV dsRNA molecule of inducing Hela cell to produce IFN and apoptosis in vitro, we carried out the Hela cell monolayer seperately with BTV and BTV dsRNA packed lipofectamine in different concentration level, and detected separately the Hela cell survival rate; the IFN content in Hela cell suspension and the cell apoptosis under the different management by MTT; Elisa Kit and flow cytometor. Analyzed the differences between BTV with different virus dosage and BTV dsRNA-lipofectamine compound with different concentration, we found that the IFN content of Hela cells produced had significant differences under above managements (p<0.05), and the ability to induce Hela cell to apoptosis of BTV dsRNA-lipofectamine compound was lower than that of BTV(p<0.05). These results manifested that BTV dsRNA could induce Hela cells to produce IFN and apoptosis, and possessed the potential to become a new IFN inducer.