高效液相色谱法测定联苯苄唑凝胶的含量

Determination of Bifonazole in Bifu by HPLC

目的:建立测定联苯苄唑凝胶(必伏)中联苯苄唑含量的方法。方法:采用高效液相色谱法(HPLC法),以甲酸-水-四氢呋喃(79∶20∶1)为流动相,检测波长为254nm。结果:联苯苄唑在0.1～5μg范围内,峰面积与进样量呈良好线性关系,平均加样回收率为100.3%(RSD=0.58%,n=9)。结论:HPLC法简便、快速、准确,能有效控制联苯苄唑凝胶的质量。

Objective:To determine the contents of bifonazole in Bifu.Methods:The analysis was carried on a column of YMC-ODS(4.6 mm×150 mm,5 μm)with a mobile of methanol-water-tetrahydrofuran(79 ∶20 ∶1).The detection wavelength was at 254 nm.Results:The linear range was 0.1～5 μg(r=0.999 9,n=6) for bifonazole.The average recovery of bifonazole was 100.3%(RSD=0.58%,n=9). Conclusion:This method is rapid,simple,accurate and good for controling the quality of the medicine.