摘要
壮观霉素产生菌４７－４号菌株经过氯化锂和紫外线联合诱变处理后，其孢子悬液分离在含有５０００μｇ／ｍｌ壮观霉素的高氏１号琼脂培养基制成的浓度梯度培养皿上。实验证明，从浓度梯度培养皿中分离得到的菌株，比用常规方法从高氏１号琼脂平板得到的菌株其壮观霉素产生能力平均高８．２％。
The spore suspension of the spectinomycin Producing mutant No.47-4 after treatment by combination of lithium chloride and ultraviolet light,was selected on the gradient plates containing 5000μg/ml spectinomycin in Gause No. 1 medium. It was proved that the strains selected from gradient plate produced average 8.2% more spectinomycinn than that ones from normal plate.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
1994年第2期58-61,共4页
Chinese Journal of Pharmaceuticals
关键词
壮观霉素
梯度培养皿
耐药株选育
spectinomycin hydrochloride, gradient plate, resistant strain selection
