基于mtDNA Cytb的六种果实蝇的分子鉴定(双翅目:实蝇科)

Molecular identification of six Bactrocera species (Diptera: Tephritidae) based on mtDNA

本研究首次对果实蝇属的桔小实蝇Bactroceradorsalis、瓜实蝇B.cucurbitae、南瓜实蝇B.tau、番石榴实蝇B.correcta、具条实蝇B.scutellata、黑漆实蝇B.scutellaris等6种实蝇mtDNACytb基因进行了测序。对这6种实蝇72个个体mtDNACytb基因中段420bp的碱基序列进行分析,得到38种单倍型,发现了116个变异位点,其中30个位点较为稳定。对这6种实蝇与其各自鉴别位点的对应关系研究表明,mtDNACytb基因可以作为这6种实蝇种类鉴别的分子标记。

The mtDNA Cytb genes of 6 fruit fly species, including Bactrocera dorsalis, B. cucurbitae, B. correcta, B. tau, B. scutellata and B. scutellaris, were sequenced. Based on the analysis of mtDNA Cytb 420 bp sequences from 72 individuals of the 6 species, we identified 38 haplotypes and detected 116 variable sites, of which 30 variable sites were highly conserved. The studies of the relationship between the 6 fruit flies and their respective identification sites suggested that mtDNA cytb sequences could be used as the molecular marker in identification of the 6 fruit fly species.