摘要
用３％海藻酸钠固定盾叶薯蓣悬浮细胞，在ＭＳ＋２，４－Ｄ１．０ｍｇ／Ｌ＋６－ＢＡ０．ｌｍｇ／Ｌ培养液中常温振荡培养较长时间后，培养液提取物经ＴＬＣ检测，表明该系统分泌出盾叶薯蓣主要成分──薯蓣皂甙元，但不能连续分泌。
he suspension cells of D.
zingiberensis were immobilized with 3% sodium alginate,and then
cultured in MS+2,4-DI. 0+ 6-BA 0. 1 at 25℃ for a long period of
time. The culture fluid free from cells was extracted and analyzed
byTLC.The result showed that the immobilized ceils could secrete the
main component of D. zingiberensis diosgen-in, but not
consecutively.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1994年第9期529-531,共3页
China Journal of Chinese Materia Medica
关键词
盾叶薯蓣
悬浮细胞
固定化
D. zingiberensis
suspension cells
sodium
alginate
immobilization
