木聚糖酶XYNB分子中折叠股B1和B2间的疏水作用对酶热稳定性的影响

Hydrophobic Interaction between β-sheet B1 and B2 in Xylanase XYNB Influencing the Enzyme Thermostability

对来源于Streptomycesolivaceoviridis的高比活木聚糖酶XYNB进行同源建模,并结合嗜热木聚糖酶氮末端芳香族氨基酸疏水作用的结构分析,设计了XYNB的T11Y定点突变,观察XYNB分子中折叠股B1和B2的疏水作用对酶的热稳定性的影响。将突变酶XYNB′在毕赤酵母中表达,表达的XYNB′经纯化后与原酶XYNB(同样经毕赤酵母表达后纯化)进行酶学性质比较,结果表明,XYNB′的耐热性比XYNB有明显的提高,但最适温度与原酶一样为6 0℃。另外,XYNB′的最适pH、Km值及比活性均有一定的改变。实验证实了木聚糖酶XYNB的氮端芳香族氨基酸之间的疏水相互作用与其热稳定性相关,为进一步的结构与功能研究提供了优良的基因材料。

A homology modeling of xylanase XYNB from Streptomyces olivaceoviridis A1 was made by Swiss-Model. The hydrophobic Interaction between β-sheet B 1and B 2 in the tertiary structure model of XYNB was compared with other thermophilic xylanase. A T11Y mutation was introduced in XYNB by site-dirrected mutagenesis to improve the thermostability of the enzyme.. The XYNB and mutant xylanase (XYNB′) expressed in Pichia pastoris were purified and their enzymatic properties were determined. The result revealed that the thermostability of XYNB′ was obviously higher than that of XYNB. The optimal temperature of XYNB′ for its activity was 60℃, similar to XYNB. But, compare to XYNB, the optimal pH value, the K m value and the specific activity of XYNB′ had also been changed.The research results suggested that the aromatic interaction between β-sheet B 1and B 2 in xylanase should increase enzyme thermostability. The mutant xylanase XYNB′ is a good material for further research in the relationship between structure and function of xylanase.