钙离子对293细胞结团和生长的影响

Effect of Calcium on Aggregation and Growth of 293 Cells

分别在有血清和无血清条件下、方瓶和转瓶中考察了Ca2 + 对2 93细胞结团和生长的影响。通过实验发现,Ca2 + 浓度在0 1～1 0mmol L范围内对2 93细胞的贴壁和结团性质有显著影响,而对生长影响不大。结果表明:有血清贴壁培养时,较高的Ca2 + 浓度有利于细胞贴壁;无血清悬浮培养中,Ca2 + 浓度越高,细胞结团越严重,细胞结团达到平衡后的平均粒径(D ,μm)与Ca2 + 浓度(c,mmol L)在0 1～0 5mmol L范围内可用一次函数D =5 8 6 5c +16 96描述,细胞结团尺寸是可调控的;而细胞在不同的Ca2 + 浓度下有相似的生长规律。

The effect of calcium on the aggregation and growth of 293 cells grown in either serum-containing or serum-free medium was investigated in T-flask and spinner bottle, respectively. It was found that the concentration of calcium ion, in the range of 0.1mmol/L to 1.0mmol/L, affected adhesion and aggregation of 293 cells severely, but had no distinct effect on growth. The result indicated that the attachment of 293 cells was easier with higher calcium concentration in serum-containing medium. And 293 cells formed aggregates readily in suspension culture. This effect was more profound in cultures with higher calcium concentration. The average diameter(D, μm) of 293 cell aggregates exhibited direct proportion to calcium concentration (c, mmol/L) in serum-free medium. It can be depicted by a simple equation in the range of 0.1mmol/L to 0.5mmol/L, i.e. D=58.65c+16.96. The aggregation size of 293 cells is regulable in suspension culture, therefore, proper control allows for an easier cell retention, and thus a high cell concentration potentially can be achieved. However, similar growth of 293 cells was observed in cultures with different calcium concentration.