Src蛋白在肺腺癌A549细胞增殖浸润中的作用

Effect of Src on proliferation and invasion of lung adenocarcinoma A549 cell line

目的:研究Src蛋白在肺腺癌A549细胞增殖浸润中的作用。方法:Westernblot检测Src蛋白在A549细胞的表达和磷酸化,MTT和Boydenchamber法分别检测抑制Src蛋白酪氨酸激酶对A549细胞体外增生和游走浸润的影响。结果:抑制Src蛋白酪氨酸激酶磷酸化,能够显著抑制A549细胞体外增生(P<0·01)和游走浸润(P<0·001)。结论:Src蛋白在肺腺癌A549细胞增殖浸润中发挥着重要作用,可能成为治疗肺腺癌的重要靶分子。

OBJECTIVE:To study the effct of Src on proliferation and invasion of lung adenocarcinoma A549 cell line. METHODS: Western blot assay were used to evaluate the expression and phosphorylation of Src in A549 cells. MTT and Boyden chamber assay were used to examine the effect of inhibition of Src phosphorylation on proliferation and invasion of A549 cells, respectively. RESULTS: Inhibition of Src phosphorylation significantly suppressed the proliferation and invasion of A549 cells, P<0.01 and P<0.001, respectively. CONSLUSION: Src plays an important role in regulating proliferation and invasion of lung adenocarcinoma A549 cells.