金华猪心脏脂肪酸结合蛋白基因的多态性分析及其与繁殖性能的关系

Investigation on Polymorphism of H-FABP Gene and Relationship with Reproductive Performances in Jinhua Pig

应用PCR-RFLP方法对金华猪Ⅰ系(164头)、金华猪Ⅱ系(41头)和金华猪Ⅲ系(52头)的心脏脂肪酸结合蛋白(H-FABP)基因的1125～1817bp和1401～2216bp段进行扩增,并用HinfⅠ、HaeⅢ和MspⅠ酶分别酶切,结果表明只有HinfⅠ存在RFLP。分析不同基因型对金华猪死产数和母仔猪初生重的影响,结果表明,H-FABP基因对经产母猪的死产数影响接近极显著(P=0.015),对头胎母仔猪初生重的影响不显著(P>0.05),对经产及所有胎次的母仔猪初生重影响极显著(P<0.01)。

The genotypes of 164 Jinhua pigs of line Ⅰ,41 Jinhua pigs of line Ⅱ and 52 Jinhua pigs of line Ⅲ for H-FABP locus were detected by PCR-RFLP. The PCR products were digested by enzyme HinfⅠ,Hae Ⅲand MspⅠ respectively. Only the HinfⅠcan cut the products.Effects of different genotypes of H-FABP on reproductive traits of Jinhua swine including stillbirth number and female birthweight were analyzed.The result showed that there was significant relationship between the genotype of H-FABP with reproductive traits of Jinhua pigs.