枯草芽孢杆菌产生在β－葡聚糖酶的研究：高产突变株CW－06的选育及其发酵条件的优化

STUDIES ON β-GLUCANASE PRODUCED BY BACILLUS SUBTILIS: SCREENING BREEDING AND OPTIMIZATION OF FERMENTATION CONDITIONS FOR MUTANT CW-06

从２２份土样中，分离得到６株β－葡聚糖酶［ＥＣ．３．２．１．７３］产生菌，经鉴定为枯草芽孢杆菌。以其中２株产酶活性较高的（６１和５５Ｕ／ｍｌ），为出发株，用ＮＴＧ诱变处理，最后得到突变株ＣＷ－０６。通过优化发酵培养基和发酵条件，使其产酶能力达到１６０Ｕ／ｍｌ以上。

Two strains of Bacillus subrilis which produce β-glucanase respectively with 61 and 55U/ml were isolated from 22 soil samples.The strains were mutated by NTG and a mutant strain CW-06 was obtained.The optimal fermentation conditions of this mutant was got by orthogonal design.In this way the β-glucanase produced by CW-06 in the broth was found more than 160 U/ml.