膀胱癌组织E-CD/cat复合体和细胞凋亡表达的临床意义

The biological significance of E-cadherinγ-catenin complex and AI in bladder cancer

目的:探讨上皮钙粘黏附素/cat(E CD/cat)复合体的表达和细胞凋亡与膀胱癌恶性程度、浸润深度及复发的关系。方法:应用免疫组织化学SABC法和DNA末端标记法(TUNEL)检测81例膀胱移行细胞癌E CD/cat复合体和细胞凋亡指数(AI)的表达情况。结果:①E CD/cat复合体的异常表达与膀胱癌临床分期、病理分级有显著关系(P<0.05),复发组E CD异常表达率(75.8%)显著高于未复发组的异常表达率(31.2%,P<0.01)。②AI与膀胱癌临床分期、病理分级与复发有显著关系(P<0.05)。③E CD正常表达组AI显著低于E CD异常表达组(P<0.01);E CD/cat复合体正常表达与AI存在负相关(r=-0.9320,P<0.01)。结论:E CD/cat复合体和AI均可作为评估膀胱癌恶性程度及预后的指标,E CD/cat复合体可能对细胞凋亡的发生起一定的抑制作用。

Objective:To determine the expression of E-cadherin-γ-catenin complexes and Apoptotic Index (AI) in bladder cancer and their relations to malignancy, infiltration and recurrence.Methods:An avidin-biotin immunoperoxidase technique and TUNEL were used to examine the expression of E-cad herinγ-catenin complexes and AI in 81 TCC biopsies.Results:①Decreased expression of E-cadherinγ-catenin complexes correlated with both high grade and stage (P<(0.05)). Moreover, abnormal E-CD expression correlated well with the high recurrence rate(P<(0.01)). ②AI correlated with both high grade and stage (P<(0.05)),AI in tumor tissues of patients with recurrence was significantly higher than that in those without recurrence(P<(0.05)). ③AI in tissues of patients with abnormal E-CD expression was significantly higher than that in those with normal E-CD expression. Statistical analysis revealed an inverse correlation between E-cadherinγ-catenin complexes and AI.Conclusions:Detection of E-cadherinγ-catenin complexes expression and AI might have practical significance in assessing the biological features of bladder cancer. E-cadherinγ-catenin complexes might be an important suppressor in some apoptosis pathways.