非小细胞肺癌中肝细胞生长因子受体蛋白表达及其与肿瘤细胞增殖、血管生成的关系

Expression of c-Met Protein in Non-small Cell Lung Carcinoma and Its Relationship with Tumor Cell Proliferation and Angiogenesis

目的:检测肝细胞生长因子受体(cMet)在非小细胞肺癌(NSCLC)中的表达,探讨其与肿瘤细胞增殖和血管形成的关系。方法:运用免疫组织化学SP法对14例正常肺组织和45例NSCLC组织中的cMet、核增殖相关抗原Ki67进行检测。用CD34单克隆抗体检测微血管密度(MVD)。结果:正常肺组织cMet表达率为21.4%(3/14),而在NSCLC中为60.0%(27/45),其在肿瘤和正常组织中的表达差异有显著性(P<0.05)。cMet的表达与组织学类型、分化程度和淋巴结转移有关(P<0.05),与年龄、性别和临床分期均无关。cMet阳性表达组的Ki67指数和MVD明显高于cMet表达阴性组(P<0.01,P<0.05),cMet的表达与Ki67指数和MVD有关。结论:cMet的表达与NSCLC的增殖和血管形成密切相关,可作为诊断和判断预后的一个指标。

Objective: To investigate the expression of hepatocyte growth factor receptor(c-Met) in non-small cell lung carcinoma(NSCLC), and to explore its relationship with tumor cell proliferation and angiogenesis. Methods: Immunnohistochemistry (SP method) was adopted to detect c-Met and ki-67 proteins expression in 14 cases of normal pulmonary tissue(NPT) and 45 cases of NSCLC. Microvessel density (MVD) was determined by anti-CD34 immunostaining. Results: In NPT, positive rate of c-Met was 21.4%(3/14), but in all of NSCLC was 60.0%(27/45). The difference of c-Met expression was significant between NSCLC and NPT(P<0.05). c-Met expression was related to differentiation degree histotype and lymph node metastasis,but not to age, sex and clinic stage.The Ki-67 indexes and MVD were significantly higher in c-Met positive tumors than those in the negative ones(P<0.01,P<0.05). The expression of c-Met was also correlated with Ki-67 indexes and MVD. Conclusion: The expression of c-Met has strong relationship with tumor cell proliferation and angiogenesis. It could serve as a diagnosable and prognostic factor of NSCLC.