摘要
目的探讨TGFβ1/Smad4信号传导系统在视网膜母细胞瘤株HxoRb44的表达及意义。方法用免疫荧光法检测TGFβ1、TGFβ受体Ⅱ及Smad4在该细胞株中的表达和定位。以已知TGFβ1、TGFβ受体Ⅱ及Smad4表达均为阳性的WI38细胞作对照,分别用RTPCR法和Westernblot法检测TGFβ1、TGFβ受体Ⅱ及Smad4的mRNA和蛋白质在视网膜母细胞瘤细胞株HxoRb44中的表达,并比较两种细胞表达水平的差异。结果免疫荧光检测结果发现视网膜母细胞瘤细胞株HxoRb44的TGFβ1呈阳性表达,主要位于胞浆和胞膜;TGFβRⅡ及Smad4阴性。视网膜母细胞瘤细胞株HxoRb44的TGFβ1的表达在mRNA水平和蛋白水平均高于作对照的WI38细胞;TGFβ受体Ⅱ及Smad4在mRNA水平和蛋白水平均为阴性。结论视网膜母细胞瘤细胞株HxoRb44高表达TGFβ1;对TGFβ1增殖抑制作用反应的丧失与视网膜母细胞瘤的发病有关。
ObjectiveTo investigate the expressions of transforming growth factor-beta1(TGF-β_1),TGF-β receptor (TGF-β R)Ⅱand Smad4 in retinoblastoma (Rb) cell line Hxo-Rb44.MethodsImmunofluorescence was used to locate the expression of TGF-β_1,TGF-β RⅡand Smad4 in cultured Rb cell line Hxo-Rb44,and RT-PCR and Western blot were used to detect the transcription and expression of TGF-β_1,TGF-β RⅡand Smad4 and the results were compared with WI-38,which served as positive control for the expression of TGF-β_1,TGF-βRⅡand Smad4.ResultsTGF-β_1 was expressed in Rb cell line Hxo-Rb44 and was mainly located in cellular membrane.The expression of TGF-β_1 was higher than that matched WI-38 cell at both mRNA and protein level.Both mRNA and protein of TGF-β RⅡand Smad4 were not expressed in Hxo-Rb44.ConclusionOverexpression of TGF-β_1 and the loss of TGF-β RⅡand Smad4 in Rb cell line Hxo-Rb44 may contribute to the formation of Rb.
出处
《眼科研究》
CAS
CSCD
北大核心
2005年第3期266-268,共3页
Chinese Ophthalmic Research
