阿法根与贝他根对体外培养人睫状上皮细胞增殖的影响

Effects of betagan and alphagan on the proliferation of human ciliary epithelial cells in vitro

目的体外培养人无色素睫状上皮(NPE)细胞,比较抗青光眼药物贝他根与阿法根对NPE细胞增殖的影响,评估两种药物使用的安全性。方法用分离法培养NPE细胞并传代,分别用4μg/mL贝他根和阿法根作用于细胞,流式细胞仪检测细胞增殖情况。通过裂解法提取细胞总蛋白,比较不同药物对细胞合成蛋白的影响。结果与对照组相比,贝他根对细胞生长周期无明显影响,而阿法根可促进S期细胞合成,对细胞生长有促进作用。两种药物都可促进细胞总蛋白合成,作用于人NPE细胞24h后,无血清对照组的总蛋白质量浓度为(2183.8±145.34)μg/mL,贝他根组的总蛋白质量浓度为(3382.82±28.10)μg/mL(与对照组相比,P=0.000),阿法根组总蛋白质量浓度为(6281.3±128.97)μg/mL。(与对照组及贝他根组相比,P=0.000)。结论两种药物对培养的NPE细胞增殖均无抑制作用,提示两种药物安全性良好。

ObjectiveTo compare the effects of two antiglaucoma drugs( levobunlol and brimonidine) on cultured human non-pigment ciliary epithelial (NPE) cells and evaluate the safety of the two drugs.MethodsNPE cells from the non-pigment layer were dissected and passaged,and 4μg/mL betagan or alphagan were added to the medium.Flow cytometry were used to detect the proliferation of cultured NPE cells.Total protein was acquired by split method,and the effect of the two drugs on the NPE cells were compared with untreated control.ResultsAs compared to the control,betagan had no significant effect on the cell cycle,while alphagan could promote the synthesis of cells in S passage,thus promoting the proliferation of cells.Both drugs increased the production of total cellular proteins.At 24 hours after treatment,the concentration of the total protein was 2183.8 ± 145.34  μg/mL in control group vs 3382.82 ± 28.10 μg/mL of betagan group(P= 0.000 ) and 6281.3 ± 128.97 μg/mL of alphagan group(P= 0.000 ),respectively.ConclusionBoth drugs do not suppress the proliferation of NPE cells,indicating their role in NPE survival.