摘要
The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\5\^9. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein（sorcin）. Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.
The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\_5\^9. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein(sorcin). Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2005年第6期1046-1048,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20273067)资助.
关键词
可溶性耐药相关钙结合蛋白
基质辅助激光解吸离子化飞行时间质谱
二维凝胶电泳
Soluble resistance-related calcium binding protein
Matrix-assisted laser desorption ionization time of flight mass spectrometry
Two-dimensional gel electrophoresis
