兔眼晶状体再生的实验研究

The experimental study on regeneration of lens

目的:观察活体内兔眼晶状体再生现象及维生表素A对晶状体再生的影响。方法:兔眼行囊晶体摘除术,术后实验给隔日腹部皮下注射维生素A0.1ml,对照组隔日腹部皮下注射用水0.1ml,注射共持续2月。术后定期观察,16周后处死动物,观察晶状体再生情况并做组织切片和超薄切片观察。结果:实验组与对照组中分别有3眼和1眼可见虹膜后再生的晶状体样物质,行光镜和电镜检查为典型晶状体结构。结论:兔眼晶体摘除后可再生出具有典型晶体结构的物质,但目前尚未观察到完整的再生晶体。维生素A对上述过程有一定的正向调节作用。

Objective:To observe the phenomena of lens regeneration in rabbits in vivo and the influence of vitamin A on it.Methods:Intracapsular lens extraction were done.Following the operation,0.1ml vitamin A oil was given by subcutaneous injection into abdomen every other day in experimental group,while 0.1ml saline was given in control group.This procedure lasted 2 months.The animals were observed regularly after operation and were put to death at the 16th week follwing the operation.The state of lens regeneration were analyzed by means of tissue sections and ultrathin sections.Results:There were 3 and 1 eye(s)in which regenerated lens-like substance was found in the back of iris respectively in experimental group and in control group.The regenerated substance was estimated to be typical lens sturcture after the light microscopical and electron microscopical analysis.Conclusion:Our experiment suggests that the substance possessing typical lens structure can be regenerated after lens extraction in rabbit,although the intact regenerated lens is not found at present.Vitamin A can accelerate this course in vivo.