蝎毒组分Ⅱ对入喉癌细胞毒性的研究

Toxicity Study of Scorpion Venom Component Ⅱ on Human Laryngocarcinoma Cells

采用细胞毒试验、肿瘤细胞杀伤试验、集落形成试验和生长抑制试验等多种方法,检测了东亚钳蝎Buthus martensil karsch蝎毒组分Ⅱ(SVC Ⅱ)对人喉痛HEp-2细胞的毒性。结果表明,以丝裂霉素为阳性对照,所用剂量范围内SVC Ⅱ(4-20μg/ml)能抑制HEp-2细胞生长和有丝分裂,对人癌细胞的毒性与药物剂量和作用时间成正相关,其中大剂量的SVC Ⅱ的抑制作用略强于MMC,提示SVC Ⅱ是蝎毒中具有抗癌活性的细胞毒素,可望成为来源于中药的新抗癌药。

By means of various methods, such as cytotxic test, killing test of tumor cell, colony forming test and growth inhibition test, the toxicity of scorpion venom componenet Ⅱ (SVC Ⅱ) of Buthus martensii karsch on HEp-2 cells of human laryngocarcinoma was detected. The results showed that, taking mitomycin (MMC) as the positive contrast, in the limits of applied dose (4-20μg/ ml) SVC Ⅱ could inhibit the growth and mitochysis of HEp-2 cells, the toxicity on human tumor cells showed positive correlation with the medicinal dose and action time. The inhibition effect of large dose of SVC Ⅱ was slightly stronger than that of MMC, which suggested that SVC Ⅱ be the cytotoxin possessing antitumor activity in SVC and might become a new kind of anticarcinogen originated from Chinese Materia Medica.