肌肽对离体培养的鸡肝细胞的影响

The Effect of Carnosine on the Chicken Hepatocytes in Vitro

研究了肌肽对离体培养的鸡肝细胞的影响。实验采用胶原酶原位二步灌流法获得鸡肝细胞,再用不同浓度的肌肽(①2 μmol L、②2 0 μmol L、③2 0 0 μmol L ,④2 0mmol L)分别进行处理。结果表明,1 )在各个时期,含2 0 μmol L肌肽的实验组肝细胞生长均好于对照组(0 μmol L肌肽) ,而且在48和72h时二者差异显著(P <0 0 5 ) ;2 ) 2 0mmol L肌肽能显著提高肝细胞的分泌白蛋白的功能(P <0 0 5 ) ;3 )添加肌肽的各实验组上清液中丙二醛(MDA)含量在2 4和48h时均低于对照组;4)添加2 0mmol L肌肽的实验组与对照组相比,能维持无血清培养肝细胞的形态达8d。表明了肌肽对鸡肝细胞增殖和分泌白蛋白有促进作用,同时可以保护肝细胞对抗过氧化,保护细胞形态。

The effect of carnosine on the chicken hepatocytes in culture was studied. The chicken hepatocytes were obtained by two steps in situ circulator perfusion method. The cell proliferation, the secretion of the albumin, and the level of the maleic dialdehyde (MDA) were determined after the addition of carnosine (0 μmol/L, 2 μmol/L, 20 μmol/L, 200 μmol/L,or 20 mmol/ L) into the culture medium. The hepatocytes cultured with 20 μmol/L carnosine proliferated better than that of control group, especially at 48 h and 72 h(P<0.05). The ability of secreting albumin (ALB) by the hepatocytes was significantly increased by 20 mmol/L carnosine. Carnosine in different doses decreased the levels of MDA. Moreover, 20 mmol/ L carnosine could keep the hepatocytes in the serum-free culture survive for more than 8 days. It could be concluded that carnosine could promote chicken hepatocyte proliferation and ALB secretion, and protect hepatocytes from peroxidation.