人体不同组织的拉曼光谱研究

Raman Spectroscopic Study of Human Tissues

测试了人体甲状腺、卵巢、输卵管、肺、子宫平滑肌、子宫颈等组织的拉曼光谱。结果显示,用氩离子激光514.5nm激发,人体组织拉曼光谱的荧光背景强,在500～3500cm-1范围,肺和宫颈组织未显示特征带,甲状腺和子宫组织则显示了较多的带。在600～1800cm-1范围,子宫、输卵管组织只显示了类胡萝卜素的拉曼带。正常甲状腺组织的光谱和甲状腺滤泡癌组织的光谱之间显示出明显差异,后者没有呈现1585和1634cm-1特征带;正常肺组织与肺癌组织的拉曼光谱也有较大区别,肺癌组织的光谱强度比正常组织的低得多。拉曼光谱技术在临床医学诊断方面将会发挥重要的作用。

Raman spectroscopy has been applied to the investigation of human thyroid, lung, oviduct, ovarian, cervical, and uterine tissues. Thyroid and uterine tissues exhibit more Raman bands than the other tissues in the range from 500 to 3 500 cm(-1) with 514.5 mn excitation, while lung and cervical tissue do not show any bands. In the range of 600-1800 cm(-1), uterine and ovarian tissues show only the Raman features of carotenoids near 1 004, 1 158, and 1 520 cm(-1). Remarkable differences between normal and follicular carcinoma samples of thyroid were observed by the absence of characteristic bands at 1585 and 1634 cm-' in the latter. The spectral intensity of lung cancerous sample is much lower than that of normal sample, by which normal and malignant samples can be discriminated: The results indicate that Raman spectroscopic technique will play an important rule in clinical diagnosing.