变形期非洲爪蛙嗅觉系统胶质原纤维酸性蛋白和波形蛋白的时空表达

THE TEMPORAL AND SPATIAL EXPRESSION OF GFAP AND VIMENTIN OF OLFACTORY SYSTEM OF XENOPUS LAEVIS DURING METAMORPHOSIS

目的研究非洲爪蛙(Xenopuslaevis)在变形期(48～63期)嗅觉系统中胶质原纤维酸性蛋白(GFAP)和波形蛋白(vimentin)的时空表达与特点。方法取发育48期(stage)到63期不同阶段幼蛙及成年蛙嗅觉系统组织,常规制成冰冻切片,选每张切片上同时包含鼻、嗅神经、嗅球的切片进行GFAP、波形蛋白等抗体的免疫荧光化学染色,用荧光显微镜观察、记录结果。结果非洲爪蛙进入变形期(48期)后,嗅神经呈GFAP IR强阳性反应,但在嗅球,仅嗅神经层为GFAP IR阳性反应,嗅小球(glomeruli)呈GFAP IR阴性反应;而波形蛋白IR阳性细胞的分布与GFAP IR阳性细胞相反,在嗅小球可见典型的波形蛋白IR阳性的放射状胶质细胞,而嗅神经却是波形蛋白阴性。结论发育过程中GFAP与波形蛋白免疫反应阳性细胞呈互补的方式分布在蛙嗅觉系统中,即GFAP IR阳性细胞主要分布在嗅觉系统的周围部分,而波形蛋白IR阳性细胞则主要分布在嗅觉系统的中央部分。

Objective To investigate features and significance of the temporal and spatial expression of GFAP and Vimentin in olfactory system of Xenopus from metamorphosis to adult. Methods Xenopus tadpoles from stage 48 to 63 were made into serial sections(20?μm)by Cryostat,each contains the nose,the olfactory nerve,and the olfactory bulb.The immunohistochemistry staining was done on these sections by anti-GFAP and anti-Vimentin,and then observed by fluorescence microscope.Results Olfactory nerve showed very strongly GFAP-IR staining during metamophosis of Xenopus.In the olfactory bulb,GFAP-IR positive staining was found only in the nerve layer,but not in glomeruli.By contrast,Vimentin-IR decorated radial glia in the olfactory bulb but faintly stained the olfactory nerve.Conclusion GFAP and Vimentin present complementary staining patterns,GFAP is expressed in the peripheral olfactory system while vimentin is expressed in the central part of olfactory system.