反相高效液相色谱法测定复方土槿皮酊中水杨酸和苯甲酸的含量

HPLC assay for salicylic acid and benzoic acid in compound hibiscus tincture

本文报道了反相高效液相色谱法测定复方土槿皮酊中水杨酸和苯甲酸含量，以对氨基苯甲酸为内标，该法简便、快速，每个样品测试在１０ｍｉｎ内完成。其中水杨酸平均回收率为１００．８５％，变异系数为０．１９％；苯甲酸平均回收率为９９．５０％，变异系数为０．２４％。

Aliquid chromatographic method was developed for the determination of Salicylic acid and Benzoic acid in compound hibis-cus tincture with p-Aminobenzoic acid as internal standard. The average recovery of Salicylic acid and Benzoic acid was foundto be 99.6% and 100.9% respectively.