摘要
应用反相高效液相色谱法测定格列甲嗪血浆浓度。色谱柱为ＯＤＳＣ１８柱，流动相为乙腈─甲醇─水（４０：２０：４０Ｖ／Ｖ），内含０．１％冰醋酸和０．０８％三乙胺。流速０．５ｍｌ／ｍｉｎ，柱温５０℃，检测波长２７５ｎｍ。线性范围在０．０２～１．００μｇ／ｍｌ（ｒ＝０．９９９９）。检测下限为０．０１μｇ／ｍｌ。平均回收率为９９．０±２．８％，ＲＳＤ＝２．８２％。日内及日间测定相对标准偏差均小于５％。
reversed phase high performance liquid chromatographic determination of glipizide in human plasma was established. Glipizide can be separated on Zorbax ODS C18 column with a mobile phase of CH3CN─MeOH─H2O(40:20:40,V/V)and de─tected at 275nm. The flow rate was5ml/min. The linear range was 0.02～1.00μg/ml(r=0.9999)and the detection lim─it was 0.01μg/ml. The average recovery was 99.05±2.79%and RSD was 2.82%. Both the coefficients of variation ofwithin─day and day─to─day were below 5.0%.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1994年第10期451-452,共2页
Chinese Journal of Hospital Pharmacy
