用反相高效液相色谱—电化学法测定血中左旋多巴和卡比多巴浓度

Determination of plasma concentration of levodopa and carbidopa by HPLC with clectro-chemical detection

本文用ＨＰＬＣ－电化学法测定血中左旋多巴（Ｌｅｖｏｄｏｐａ）和卡比多巴（Ｃａｒｂｉｄｏｐａ）浓度，并对家兔体内药动学进行了初步研究。结果表明：两药在２５－６０００ｎｇ／ｍｌ范围内，峰高与浓度具有良好的相关性（两药均为ｒ＝０．９９９３），最低检测限均为０．４ｎｇ。日内变异系数小于１２％，日间变异系数小于１５％。回收率左旋多巴为９７．６７％，卡比多巴为８８．５５％。家兔口服复方多巴片后，药时曲线为一房室模型，左旋多巴半衰期为２．２７ｈ，卡比多巴半衰期为３．２２ｈ，与国外文献报道基本一致。用ＨＰＬＣ－电化学法测定血中左旋多巴和卡比多巴脓度，为帕金森氏病的合理用药提供一种稳定、可靠的重要工具。

he plasma concentration of levodopa and carbidopa was determined by Hight-performance liquid chromatography withelectro-chem ical detection simultaneously , and the pharmacokinetic of levodopa and carbidopa in rabbit was studied. The re-sults showed that the concentration range was 25-6000ng/ml(levodopa r= 0.9992, carbidopa r= 0. 9993). The detection lim-it was 20ng/ml. The RSD of within-day and between- day were respectively lower than 12%and 15%,The mean recoverieswere 97.6%(levodopa) and 88. 55%(carbidopa).An open model of one compartments was the best approach for the phar-macokinetic profile of levedopa and carbidopa administered orally to the rabbits. The biological half-lifes (t1/2)of levedopaand carbidopa respoctively 2. 27h and 3. 22 h , it was similar to external references. The determination of levodopa and car-bidopa in plasma offered a sensitive reliable assay method for the treatment of Parkinson' s disease.