摘要
在无血清和含1.0%、5.0%血清培养的新生大鼠心肌细胞标本上,去甲肾上腺素(NE,2.0μmol/L)使细胞蛋白质含量(Lowry法)分别比相应对照组增加40%、26%、19%(P均<0.01);细胞3H-亮氨酸参入量与NE浓度呈正性剂量依赖性,最大效应浓度为20.0μmol/L;无血清培养体系中,0.2、2.0、20μmol/L的NE使参入量分别比对照增加17.8%、353%、37.7%;1.0%血清培养体系中分别比对照增加16.2%、27.9%、31.6%(P均<0.05);哌唑嗪(2.0μmol/L)可阻断NE的促蛋白质合成作用,心得安(2.0μmol/L)则无此效应。提示在无血清或低浓度血清培养体系中,NE可促进心肌细胞蛋白质合成,增加细胞蛋白质含量,该作用可能是通过α1肾上腺素能受体介导。
The effects of α1-adrenergic stimulation on the rate of protein synthesis and the protein con-tent in primary cultures of neonatal rat cardiomyocytes were examined. Treatment ofcardiomyocyte with noradrenaline(NE, 2.0μmol /L) increased total cellular protein content by40%, 26%and 19%(P<0.01) respectively in the serum-free, 1.0% and 5.0% serum-supplementmedia as compared with the control. Addition of NE to the serum-free medium or the mediumwith 1.0% serum-supplement indueed a dose-dependent increase in 3H-leucine incorporationinto cardiomyocyte protein. After addition of0.2,2.0,20.0μmol/L NE, the 3H-leucine incor-poration was increased by 17.8%, 35.3%, 37.7%(P<0 .01) respectively in the serum-free me-dium and by 16.2%, 27.9%,31.6% (P<0.01)in the medium with 10% serum-supplement.Theselective α1-adrenergic antagonists prazosin(2.0μmol/L), but not β-adrenergic blockerpropranolol(2.0μmol/L), effectively inhibited the NE-induced increase in either total cellularprotein or 3H-leucine incorporation. These results suggest that α1-adrenergic stimulation in-creases protein content of cardiomyocytes by accelerating protein synthesis.
出处
《中国应用生理学杂志》
CSCD
1994年第1期13-17,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金
关键词
蛋白
合成
心肌细胞
去甲肾上腺素
protein synthesis
cultured cardiomyocyte
noradrenaline
adrenergic receptors
neonatal rat