摘要
将分散的新生大鼠皮层神经元种在预先已培养了１０－１４ｄ的胶质细胞上，可使接种密度降至２．５×１０５／ｍｌ，同时细胞呈分散状生长，利于进行电生理学研究。采用膜片钳技术，在膜片钳放大器上记录了培养神经元的电生理特性，证明在未实验提供的培养条件下，神经元生长良好，保持了很好的生理功能。
Newborn rat
cortical neurons were grown in dissociated cell culture.Cortical cells plated ona monolayer of
confluent glia grew at a low density(about 2.5 × 105cell/35mm dish)and showedvery low
tendency of reaggregation.Voltage-activated Na+and K+currents,γ-aminobutyricacid-and
glutamate-induced currents,and spontaneous synaptic current could be recorded bywhole cell
voltage-clamp mode.The results provide evidence that rat neurons grown in vitro canexpress
similar electrophysiological properties to their counterparts in vivo, thus cell cultureproves to
be a useful tool to study cell function.
出处
《中国应用生理学杂志》
CAS
CSCD
1994年第2期179-182,共4页
Chinese Journal of Applied Physiology
关键词
新生大鼠
电生理
神经元
培养
方法
newborn rat
cortical cell culture
whole-cell
recording
electrophysiology
