稻秸半纤维素水解条件和水解液脱毒的研究

Study of Hemicellulose Hydrolysis of the Rice Straw and Hydrolysate Detoxification

为了充分利用稻秸半纤维素,并提高水解液发酵性能,对稻秸半纤维素的水解条件和水解液脱毒进行了研究。考察了酸浓度、温度、时间和固液比对稻秸半纤维素水解率的影响;通过正交实验设计确定水解的最优条件为:硫酸质量分数3%,温度120℃,时间1.5h,固液比(W/V)1∶10;在此条件下得到水解液,通过对有机溶剂萃取、大孔树脂吸附和活性炭吸附多种脱毒方法的比较,确定用Ca(OH)2+S-8型树脂进行水解液脱毒。

Hydrolysis condition of hemicellulose in the rice straw and the hydrolysate detoxification are studied to utilize sufficiently hemicellulose and improve hydrolysate fermentability. Sulfuric acid is employed as catalyzer. The influence of sulfuric acid concentration, reaction time, hydrolysis temperature and solid to liquid ratio on hydrolysis efficiency of hemicellulose is considered. The optimal hydrolysis condition established by perpendicular design is sulfuric acid concentration 3%, reaction time 1.5 h, hydrolysis temperature 120℃, solid to liquid ratio(W/V) 1∶10. Several hydrolysate detoxification methods are compared which are organic solvents extraction, adsorption resins and activated charcoal. Method of overliming with calcium hydroxide followed by S-8 adsorption resin treatment obtains the optimal detoxification effect.