人肝刺激因子对大鼠实验性慢性肝损伤的保护作用

PROTECTIVE EFFECT OF HUMAN HEPATIC STIMULATOR SUBSTANCE ON EXPERIMENTAL CHRONIC LIVER INJURY IN RATS

从健康孕妇水囊引产４─６个月龄的胎儿取肝，采用ＬａＢｒｅｃｑｕｅ方法提取人肝刺激因子（ｈＨＳＳ）。经３Ｈ－胸腺嘧啶核苷参入肝ＤＮＡ法测定其生物活性。表明此ｈＨＳＳ可刺激肝细胞ＤＮＡ合成。采用皮下注射ＣＣｌ４和饮用１０％乙醇来制备慢性肝损伤动物模型，观察了ｈＨＳＳ的保护肝脏作用。结果表明：ｈＨＳＳ可使ＣＣｌ４－乙醇所致慢性肝损伤大鼠的死亡率、血清谷丙转氨酶水平、肝组织中羟脯氨酸含量的升高以及肝组织中丙二醛的含量降低。肝组织切片表明：ｈＨＳＳ能减轻肝组织的损伤程度，促进肝细胞再生，并能明显防止肝纤维化的形成和发展。可见，ｈＨＳＳ对ＣＣｌ４－乙醇所致的慢性肝损伤大鼠有明显的保护作用，其机制可能与促进肝细胞再生及抑制肝细胞膜的脂质过氧化有关。

Human hepatic stimulator substance (hHSS) was extracted from the liver of 4─6 month fetus from healthy pregnant women according to the method of LaBrecque. The bioactivity of hHSS was determined by measurement of the rate of 3H-thymidine incorporation into the hepatocyte DNA after injection of hHSS into the abdominal cavity of partial hepatectomized rats. The results showed that hHSS could stimulate the synthesis of hepatocyte DNA (P<0.01).The model experimental liver injury established by hypodermic injection of carbon tetrachloride and feeding ethanol. The results showed that hHSS could significantly lower the mortality , suppress the elevation of serum ALT, lessen the elevation of liver hydroxyproline (Hyp) content and lower the elevated liver MDA content in the CCl4-ethanol intoxicated rats.Histological examination indicated that hHSS could lessen the degree of degeneration and necrosis of the hepatocytes, stimulate the regeneration and prevent the formation and progression of hepatic fibrosis.