摘要
测定油菜籽硫甙总量的内源酶法．其原理是依据内源硫甙酶水解硫甙，以３，５一二硝基水杨酸为显色剂，测定水解产物葡萄糖量，再换算成硫甙总量。４０％的酸化甲醇可完全抑制硫甙酶活性．方法简单、快速，精密度高，重现性好。葡萄糖回收率为９３．６４％±１．３７％。
Glucosinlotes in rapeseed hydrolysed with endogenous myrosinaes to liberate glucose,and then glucose was measured by 3. 5-dinitro salicylic acid colorimetric procedure.The method was simple .rapid .repoducible and the addition of exogenous myrosinase was unnecessary. Addition of glucose to the digestion mixtures gave 93. 64±1. 37% recovery. It gave figures in excellent agreement with glucoxidase/peroxidase method.
出处
《中国油料》
CAS
CSCD
北大核心
1994年第4期46-49,共4页
关键词
油菜籽
硫甙总量
内源酶
3.5一二硝基水杨酸
Rapeseed
Total glucosinolate content
Endogenous enzyme
3, 5-dinitro salicylic acid
