花生去子叶幼胚的丛生芽诱导和植株再生

EFFECTIVE PLANT REGENERATION VIA ORGANOGENESIS FROM DECOTYLEDON-IMMATURE EMBRYO CULTURE IN PEANUT (Arachis hypogaea)

授粉后２５－３０天的花生幼胚，去子叶后，在ＭＳ２（ＭＳ＋ＢＡ１．０ｍｇ／Ｌ＋ＣＨ３００ｍｇ／Ｌ＋ＰＶＰＰ０．３％＋椰计５０ｍｌ／Ｌ＋蔗糖４％＋琼脂０．８％）培养基中，置３００ｌｕｘ弱光和２０℃±１℃条件下，培养２１－２８天，能有效地产生丛生芽；在ＭＳ２中诱导培养后的去子叶幼胚的生长点切去，在改良无激素培养基（ＭＳ１）中培养１４天，转入ＭＳ３（ＭＳ＋ＢＡ６．０ｍｇ／Ｌ＋ＮＡＡ０．４ｍｌ／Ｌ＋Ｄ－生物素０．１ｍｇ／Ｌ＋ＣＨ３００ｍｇ／Ｌ＋酵母浸出物２００ｍｇ／Ｌ＋椰计５０ｍ／Ｌ＋ＰＶＰＰ０．３％＋蔗糖２．５％＋琼脂０．８％）培养基，置２５℃±１℃，３５００ｌｕｘ光照条件下培养２１－２８天，８０％的外植体分化出丛生芽，继而长成无根带叶小苗．．平均每外植体产生８．１个丛生芽，最多可达４０个．诱根后小苗移植田间８０％植株成活并开花结实。品种（系）间差异不显著。

An experiment using five peanut cultivars was conducted for plant regeneration via organogenesis from decotyledon-immature embryo cultuer. The decotyledon young embryoes were cultured on the induction medium (Modifed MS with BA 1. 0mg/IL+CH300mg/L+PV PP 0. 3%+coconut milk 50mL /L+sucrose4%+agarose0. 8 %, PH =5. 8)under dark of dim light at 20℃±1℃for21-28 days. The embryo germinating point was cut out or killed and the explants were then transfered to a secondary medium (with BA 0. 6mg/L+NAA 0. 4mg/L+biotin 0.1mg/L+CH 300mg/L+yeast extract 200mg/L+coconut milk 50mL/L+PV PP 0. 3%+sucrose 2. 5%+agarose 0. 8% .PH=5. 8)and cultured for 28 days.The rate of polyshoot-organogenesis explants was about 31% .and the average number ofshoots per explant was 6. 7. Roots were well induced on the root-inducing medium. The regenerated plants were tranfered to field plot and seeds from these plants were obtained. No significant difference in regeneration ratio was observed among the five cultivars used.