免疫散射比浊法测定CRP

Nephelometry in detecting serum CRP concentration

目的本文对比浊法测定血清CRP(C反应蛋白)进行方法学评价。方法采用免疫散射比浊法检测100例患者和30例健康正常人血清CRP含量,同时与乳胶凝集法结果对比。结果批内、批间变异系数分别为1.41%和4.66%,定量检测具有满意的线性响应(r=0.987),免疫比浊法阳性率为35%,乳胶法为18%,比浊法阳性率明显高于乳胶法。结论本方法的重复性、准确性、稳定性、特异性均较好,脂血、黄疸无明显影响,具有较高的临床推广价值。

Objective Methodological evaluation of nephelometry was performed in the detection of serum CRP. Methods The levels of serum CRP in 100 patients and 30 normal subjects were determined by two methods: nephelometry and latex agglutination test, and the results were compared. Results The CV value of intra-assays of CRP was 1.41%, and that of inter-assays of CRP was 4.66%. Quantification detection shows a satisfactory line relation (r=0.987). The positive rate of CRP was 35% by nephelometry, and 18% by latex agglutination test. A higher positive rate was obtained by nephelometry in comparison with latex latex agglutination test. Conclusion Nephelometry is quite good in replication, accuracy, stability, and specificity, and not affected by lipid and jaundice. Nephelometry is valuable in clinical application.