摘要
目的:观察D-氨基葡萄糖衍生物体外对人胃癌SGC- 7901细胞增生的影响,探讨其可能作用机制. 方法:采用MTT法,筛选药物作用最佳浓度.用流式细胞仪分析诱导凋亡前后的细胞DNA含量,透射电镜观察凋亡细胞的超微结构.免疫组化测定CD44表达. 结果:D-氨基葡萄糖衍生物能明显抑制胃癌细胞的增长(P<0.01),MTT法显示抑制程度具有时间和剂量效应关系,统计组间比较差异有显著性(P<0.01);流式细胞仪分析可见亚二倍体(Sub-G1)凋亡峰;电镜观察到凋亡细胞的典型变化,免疫组化及光密度检测示CD44表达下降(216.5±7.0 vs 190.0±14.2,P=0.000). 结论:D-氨基葡萄糖衍生物通过诱导肿瘤细胞凋亡而抑制人胃癌SGC-7901细胞增生,同时还有下调CD44的作用.
AIM: To study the in vitro inhibitory effect of amino-D-glu-cose derivative on the proliferation of the gastric carcinoma cell line SGC-7901. METHODS: Cell growth was measured by MTT colorimet-ric assay. DNA content was detected by flow cytometer (FCM). The morphological changes of apoptotic cells were evaluated by transmission electronic microscopy. CD44 expression was analyzed by immunohistochemistry. RESULTS: Amino-D-glucose derivative inhibited SGC-7901 cell in a time- and dose-dependent manner. After treatment, apoptosis peak was demonstrated by FCM, and apoptotic morphological changes were observed under microscope. Immunohistochemist and image analysis showed that expression of CD44 was downregulated (216.5±7.0 vs 190.0±14.2, P= 0.000). CONCLUSION: Amino-D-glucose derivative can induce SGC -7901 cell apoptosis, inhibit the cancer proliferation and downregulate expression of CD44 expression.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第6期720-723,共4页
World Chinese Journal of Digestology
