摘要
用PCR技术从油桐尺蠖核型多角体病毒基因组中扩增得到p35基因的DNA序列,把PCR产物克隆到pMD 18载体上,核苷酸序列测定表明此片段与AcNPVp35基因同源性为100%,继而构建了表达质粒pGBp35,诱导后经SDS PAGE检测表明获得了BsNPVp35基因在大肠杆菌BL21中包涵体形式的特异表达。
A 940bp fragment was produced by PCR from Buzura suppressaria nuclear polyhedrosis virus DNA and sequenced after cloned into plasmid pMD-18. We have cloned and expressed the gene in Escherichia coli cells and got high level yielding of protein. The protein have been assayed by the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
出处
《中国生物防治》
CSCD
北大核心
2005年第2期99-103,共5页
Chinese Journal of Biological Control
基金
南京师范大学科研基金(211100G024)
