日本血吸虫大陆株粘蛋白样蛋白部分基因的克隆

Cloning of the gene coding for mucin-like protein from chinese Schistosoma japonicum

目的构建日本血吸虫中国大陆株粘蛋白样蛋白(SjMLP)核酸疫苗。方法分子克隆常规操作将扩增产物 SjMLP 编码区基因序列克隆至载体 pUCm-T 中,然后亚克隆到真核表达载体 pcDNA3.1(+)中。结果经酶切、PCR 及测序鉴定表明所构建的质粒 pUCm-T/SjMLP 和 pcDNA3.1(+)/SjMLP 中含有所扩增的基因序列。结论成功地构建了含目的基因的真核表达质粒 pcDNA3.1(+)/SjMLP,从而,为进一步对其进行 DNA 免疫系列研究工作奠定了基础。

Objective To clone the part gene coding for schistosoma japonicum mucin-like protein into the eukaryotic expression vector pcDNA3.1(+)and to construct a DNA vaccine.Methods TThe fragment of the SjMLP was amplified by PCR and cloned into cloning vector pUCm-T,then it was subcloned into eukaryotic expression vector pcDNA3.1(+).Results The coding region of SjMLP gene was verified after tested by PCR and by sequencing.Conclusion The eukaryotic expression plasmid was successfully constructed,which pro- vided the basis for further study on DNA immunization.