超声破坏微泡治疗大鼠下肢血管闭塞的研究

Study of treatment of vascular occlusion by ultrasound-mediated microbubble destruction in the rat hindlimb

目的:用超声破坏微泡的方法促进大鼠下肢血管闭塞模型的血管新生。方法: 30只大鼠随机分为3组,建立下肢血管闭塞模型,一组采用超声破坏微泡的方法;一组单纯超声作用;一组作为对照。14天后处死各组大鼠,用免疫组织化学方法观察骨骼肌组织中血管内皮生长因子(vascularendothelialgrowthfactorVEGF)的表达和血管新生情况。结果:用超声破坏微泡的方法可在大鼠血管闭塞模型中促进VEGF的表达和缺血骨骼肌的血管再生,与单纯超声组和对照组比较有显著性差异。结论:超声破坏微泡可促进血管闭塞后骨骼肌中VEGF的表达和血管新生。

Objective: To explore whether ultrasound-mediated microbubble destruction could augment the revascularization in the rat ischemical hindlimb.Methods: hindlimb ischemia was formed in 30 healthy Wistar rats after they were randomly divided into 3 groups. In group one, ultrasound irradiation was applied on the ischemic hindlimb after intravenous injection of ultrasound contrast agent at a dose of 0.2 ml. In group two ultrasound irradiation was simply applied on the ischemic hindlimb.In group three, the same dose of saline was injected through the tail vein as control.14 days after the treatment,the hindlimbs were harvested for Immunohistochemistry stain of VEGF and factor Ⅷ, and microvessel density(MVD) was observed. Results: There was significant VEGF and factor Ⅷ expression in group 1, little VEGF and factor Ⅷ expression was seen in group 2 and 3; and there was significant difference on the MVD among the 3 groups. Conclusion: Ultrasound-mediated microbubble destruction could accelerate the anguogenesis in rat ischemical hindlimb, it provides a new method for peripheral vascular occlusive disease.