丹皮酚注射液增强免疫功能的实验研究

A Study of Paeonol Injection on Immune Functions in Rats

丹皮酚在低浓度时（１０～１５ｍｇ／ｋｇ）能够显著提高外周血酸性α－醋酸萘酯酶（ＡＮＡＥ）阳性淋巴细胞百分率和白细胞移行抑制因子的释放，从而增强机体细胞免疫功能：并且能够显著改善外周血中性白细胞对金黄色葡萄球菌的吞噬作用，而增强机体非特异性免疫功能。丹皮酚对于脾脏特异性玫瑰花环形成细胞的比率虽有所提高，但在统计学上意义不显著。

Paeonol is the main effective component of the traditional Chinese medicine,Paeonia suffroticosa.Paeonol has some therapeutic effects in resisting many species of bacteria and fungi.But the mechanism of such effects is still unclear.We used low dosage of paeonol injection(10-15 mg/kg)to observe its effect on the functions of the immune system of the Wistar's rats.The methods include peripheral blood ANAE+ lymphocytes(PBAL),leucocyte migrating inhibition factors(LMIF),specific rosette forming cells(SRFC)of spleen,and bacteria phagocytoses of polymorphonuclear neutrophil(BPP).The results showed that paeonol could enhance specific cellular immunity by raising the percentage of the PBAL(P<0.01)and the release of LMIF(P<0.01).Paeonol could also increase the BPP(P<0.01).On the other hand,although it could increase the percentage of SRFC of the spleen,such an effect showed no significant difference(P>0.1).These enhancing effects of the specific cellular immunity and the nonspecific phagocytosis functions of the immune system might be one of the mechanisms of the antibacterial effects of paeonol.The possible modulating activity of paeonol may develop a new applied field in clinical practice.