毛细管电泳法快速检测消毒剂中的醋酸氯己定

Determination of Chlorhexidine Acetate in Disinfectors by Capillary Electrophoresis

建立了消毒剂中活性成分醋酸氯己定(又名:醋酸洗必泰)的毛细管电泳快速检测法。采用15mmol/L磷酸盐乙腈(体积比为60∶40)缓冲体系,将醋酸氯己定在50cm×75μmi.d.的石英毛细管柱中进行电泳分离,电泳电压为15kV,检测波长为254nm。同时,对毛细管电泳分析醋酸氯己定的条件(如缓冲液的种类、pH值、浓度及电泳电压等)进行了优化。用该方法对消毒剂样品进行测定,在4min内可完成分析。醋酸氯己定在质量浓度为0.01～0.10g/L时线性良好,检测限为0.004mg/L,吸光度值的相对标准偏差为3.97%,迁移时间的相对标准偏差为2.99%,样品加标回收率为91.4%～116.6%。将该方法与高效液相色谱法进行比较,两种方法测定结果的相对误差≤4%。所建立的检测醋酸氯己定含量的毛细管区带电泳法简单、快速,适用于消毒剂样品的测定。

A method for the determination of chlorhexidine acetate in disinfectors using capillary electrophoresis (CE) was developed using the following conditions: detection wavelength, 254 nm; applied voltage, 15 kV; uncoated fused silica capillary column, 50 cm×75 μm i.d.; buffer, 15 mmol/L phosphate and acetonitrile (60∶40, v/v). Under the optimum conditions, chlorhexidine acetate in disinfectors was determinated in 4 min. The effects of different parameters, such as buffer component, buffer concentration, buffer pH value and electrophoretic voltage, on the CE analysis of chlorhexidine acetate were studied in detail. The linear range of the proposed method was 0.01-0.10 g/L. The detection limit was 0.004 mg/L. The relative standard deviation (RSD) of absorbance was 3.97% and the RSD of migration time was 2.99%. The spiked recoveries of samples were 91.4%-116.6%. The method was compared with the high performance liquid chromatographic method and the relative error was less than 4.0%. The proposed method was simple, quick and suitable for disinfector analysis.