三七总皂甙抗炎和镇痛作用及其机理探讨

Effects and Mechanism of Total Saponins of Panax Notoginseng on Anti-Inflammation and Analgesia

本研究通过建立炎症动物模型，应用三七总皂甙（ＴＳＰＮ）、电针（ＥＡ）进行治疗，并用纳洛酮（Ｎｘ）阻断两者作用，用组织化学的方法对ＴＳＰＮ和ＥＡ的效应进行比较。结果可见痛阈（ＰＴ）在ＴＳＰＮ组、ＥＡ组和ＥＡ加ＴＳＰＮ组均显著提高，且硝基蓝四氮唑试验（ＮＢＴ）阳性的多形核白细胞（ＰＭＮ）和醋酸萘酚酯酶（ＡＮＡＥ）的点型亚群计数均明显增加，以上的全部效应均被纳洛酮部分阻断；ＴＳＰＮ组与Ｎｘ组腹腔肥大细胞脱颗粒率无明显差异。由此可见，ＴＳＰＮ与ＥＡ效应相似，具有明显的抗炎镇痛及免疫调整作用。这提示三七总皂甙可能是阿片样肽受体的激动剂而不具有成瘾副作用。

In this study the effects of total saponins of Panax notoginseng(TSPN)and electroacupuncture(EA)were compared.Liquid paraffin was intraperitoneally injected (0.1ml/mouse)to establish the animal model with inflammation.The mice were randomly divided into 4 groups with different treatments for 7 days:EA group,TSPN group(100mg.kg intraperitoneal administration),Naloxone(Nx)plus TSPN group and EA plus TSPN group.The pain threshold was measured by a detector(EQ-9E)and the nitroblue tetrazolium test(NBT)for polymorphonuclear neutrophil(PMN)bacteriocidal activity and the α-naphthyl acetate esterase(ANAE)histochemical staining for detection of the focal pattern lymphocyte subpopulation and the toluidine blue histochemical staining for detection of degranulation rate peritoneal mast cells were performed.The results showed that in TSPN,EA and EA plus TSPN group the pain threshold was elevated significantly,the enumeration of NBTpositive PMN and the ANAE-F lymphocyte subpopulation was enhanced.All the above effocts could be partially inhibited by naloxone.Between TSPN group and Nx group the degranulation rate of peritoneal mast cells had no significant difference.Since the TSPN and EA have similar effects e.g.anti-inflammatory,analgesic and immunomodulatory action,it suggested that the TSPN might be somewhat agonist of the opioid like peptide receptor without addiction side reactions.