高效液相色谱法同时测定制药废水中的交沙霉素、茶碱及扑热息痛

Simultaneous Determination of Josamycin, Theophylline and Paracetamol in Pharmaceutical Waste Water by High Performance Liquid Chromatography

建立了同时测定制药废水中残留的交沙霉素、茶碱、扑热息痛等3种药物的高效液相色谱方法。样品经固相萃取处理后进行色谱分析。采用的色谱条件:色谱柱为HypersilODS柱(4.6mmi.d.×200mm);流动相A液为0.025mol/LKH2PO4H3PO4缓冲液(pH2.75),流动相B液为甲醇;梯度洗脱;紫外检测波长为230nm(交沙霉素)、272nm(茶碱)、243nm(扑热息痛)。制药废水中3种药物的加标回收率均高于93%,相对标准偏差(n=6)小于2.1%,检测下限(S/N=3)不高于1.0μg/L。该方法已应用于制药废水的生物强化降解研究。

A high performance liquid chromatographic (HPLC) method was established for the simultaneous determination of three main pollutants (josamycin, theophylline and paracetamol) in pharmaceutical waste water. The three drug residues were concentrated with a SepPak C 18 solid-phase extracted column, and eluted with methanol. The HPLC separation was performed on a Hypersil ODS column (200 mm×4.6 mm i.d.) with a gradient eluting system of 0.025 mol/LKH 2PO 4-H 3PO 4 (pH 2.75)-methanol. The detection wavelengths were 230 nm for josamycin, 272 nm for theophylline, and 243 nm for paracetamol. The linear ranges of the drugs were 0.1-100 mg/L with correlation coefficients between 0.9993 and 0.9995. The recoveries were more than 93%, and relative standard deviations (n=6) were less than 2.1%. The detection limits (S/N=3) were less than 1.0 μg/L.