黄芪对小鼠急性病毒性心肌炎作用的心肌细胞电生理研究

Effect of Astragalus Membranaceus on Electrophysiological Activities of Acute Experimental Coxsackie B_3 Viral Myocarditis in Mice

本实验应用常规细胞内微电极技术及实时微机数据处理系统记录心肌细胞电活动参数：静息电位（ＲＰ）、动作电位振幅（ＡＰＡ）、超射（ＯＳ）、动作电位零相最大除极速率（Ｖｍａｘ）、动作电位时间（ＡＰＤ５０和ＡＰＤ９０）和心肌细胞异常波形动作电位，研究了不同时期使用黄芪对急性实验性小鼠柯萨奇Ｂ３病毒（ＣＢ３Ｖ）性心肌炎模型心肌细胞异常电活动的影响。结果发现在感染病毒同时和感染病毒后３天开始每天给小鼠腹腔注射黄芪（每只０．８ｇ／０．４ｍｌ）１周，能使心肌炎小鼠心肌细胞异常电活动得到部分改善；而感染病毒前１天１次性腹腔注射黄芪（每只０．８ｇ／０．４ｍｌ）对心肌炎小鼠心肌细胞异常电活动没有影响。提示早期使用黄芪对急性ＣＢ３Ｖ性心肌炎有一定防治作用，单剂量黄芪对急性ＣＢ３Ｖ性心肌炎没有预防作用。

A murine model for observing the effect of Astragalus membranaceus (AM) on electrophysiological activity of the right ventricular myocardium was developed in 4 week-old male BALB/c mice infected with Coxsackie B3 virus (CB3V).The conventional microelectrode technique and real-time microcomputer data processor system was used.The survival rate in infected-AM treated mice was significantly higher and the percentage of abnormal action potential was much lower than those in control mice (P<0.05 and <0.01 respectively).Some abnormal electrophysiological parameters,such as APA,OS and Vmax in infected myocardium were found to be improved by AM treatment.Single dose of AM did not show beneficial effect in murine myocardium infected with CB3V.The results suggested that AM might be valuable in the prevention and treatment of acute myocarditis invloving Coxsackie B3 virus.