胰腺癌和慢性胰腺炎趋化因子的表达及其意义

Study on the expressions of chemotactic proteins and their significance in pancreatic carcinoma and chronic pancreatitis

目的 目的探讨胰腺癌和慢性胰腺炎组织中白细胞介素8 (IL 8 )、单核细胞趋化蛋白1(MCP 1 )和巨噬细胞炎性蛋白1α(MIP 1α)的表达及其生物学意义,并探讨三者在胰腺癌中表达的相互关系。方法 采用ABC免疫组织化学法检测5 1例胰腺癌和1 0例慢性胰腺炎手术切除标本的上述各项指标。结果 胰腺癌IL 8,MCP 1和MIP 1α表达阳性率及其评分明显高于慢性胰腺炎(P< 0. 0 1 )。高分化腺癌的IL 8显著高于低分化腺癌(P < 0. 0 5 );未转移癌三者阳性率及评分明显低于伴转移病例(P< 0. 0 5或P < 0. 0 1 )。结论 IL 8,MCP 1,MIP 1α三者表达均为反映胰腺癌发生发展、生物学行为、转移发生及其预后的重要化学趋化因子;三者可能存在着相互影响和共同调控的作用途径。

Objective To investigate the expressions of IL-8,MCP-1 and MIP-1α and their biological significance in pancreatic carcinoma and chronic pancreatitis, and investigate the relationships of their expressions in pancreatic cancer tissues.Methods Immunohistochemical method of avidin-biotin complex was used to examine surgically removed specimens from 51 cases of pancreatic carcinoma and 10 cases of chronic pancreatitis. Results The positive rates of expression and the scorings of IL-8,MCP-1,MIP-1α were significantly higher in pancreatic carcinoma than that in chronic pancreatitis(P<0.01). IL-8 expression was significantly higher in well-differentiated adenocarcinoma than that in poorly-differentiated ones(P< 0.05 ). Also the positive expression rate and the scorings of the three factors were significantly lower in cases without metastasis than in those with metastasis(P<0.05 or P<0.01).Conclusions The expressions of IL-8,mcp-I and MIP-Iα might be three important chemotactic factors in reflecting the carcinogenesis, progression ,biological behavior,metastatic potential and prognosis of pancreatic carcinoma.The three factors might have mutual effect and common regulatory pathways of action.