皮肤恶性黑素瘤凋亡相关基因Survivin Bax表达及其与端粒酶的关系

Expression of Survivin Bax and hTERT in Cutaneous Malignant Melanoma and the Relationship Between Them

目的探讨凋亡相关基因Survivin,Bax及人端粒酶逆转录酶(hTERT)在皮肤恶性黑素瘤中的表达及其临床意义。方法SP免疫组化染色检测63例皮肤恶性黑素瘤和21例色素痣组织中Survivin,Bax蛋白水平;原位杂交技术检测上述组织中人端粒酶逆转录酶(hTERT)mRNA水平。结果①凋亡相关蛋白Survivin在皮肤恶性黑素瘤中的表达明显强于其在色素痣中的表达,Bax在二者中的表达无明显差异,hTERTmRNA表达与Survivin相似;②Survivin蛋白和hTERTmRNA表达均与恶性黑素瘤病理学类型、淋巴结转移、浸润深度及预后相关,Bax表达与之无相关;③hTERTmRNA在黑素瘤中的表达水平与Survivin呈高度正相关,与Bax表达无关,Survivin与Bax表达水平无明显相关性。结论皮肤恶性黑素瘤形成和发展与肿瘤细胞抗凋亡(Survivin)和促进增殖(hTERT)均密切相关;Survivin和hTERT对皮肤恶性黑素瘤的辅助诊断及预后评估具有重要的临床意义;Bax表达与皮肤恶性黑素瘤的发生发展无明显相关性。

Objective To investigate the expression of survivin,Bax and hTERT(human telomerase reverse transcriptase)and their clinical significance in cutaneous malignant melanoma(CMM).Methods The expression of survivin and Bax was measured by streptavidin-peroxidase complex immunohistochemical technique in 63 cases of CMM and 21 cases of benign nevi;The expression of hTERT mRNA was measured by in situ hybridization technique.Results The positive expression percentage of survivin and hTERT in CMM was significantly higer than that of benign nevi,while the positive expression percentage of Bax in that was similar.The expression of survivin and hTERT in CMM was positively related to pathological histology,Clark’s grade,the metastasis to lymph nodes and clinical outcome,while no relationship was observed between the expression of Bax and pathological histology,Clark’s grade,the metastasis to lymph nodes and clinical outcome in CMM.The expression of hTERT was positively correlated with the high expression of survivin but not with that of bax in CMM.The expression of survivin was also not correlated with that of Bax.Conclusion Survivin and hTERT might play important roles in the carcinogenesis process and progression of CMM and have important implications in the diagnosis and prognostic assessment.The expression of Bax have no significant relationship with the carcinogenesis process and progression of CMM.