三氧化二砷纳米粒体外诱导人肝癌细胞凋亡的研究

Research of human liver cancer cell apoptosis induced by As_2O_3 nanoparticles

目的:研究三氧化二砷(As2O3)新剂型纳米粒的制备方法及体外诱导肝癌细胞凋亡的作用,并与传统的As2O3溶液进行比较。方法:①采用溶胶凝胶法制备As2O3纳米粒;②用光镜、电镜、MTT、流式细胞仪法研究As2O3纳米粒诱导人肝癌细胞凋亡的效果。结果:①制备了大小约为80nm和40nm两种粒径的As2O3纳米粒子,电镜下呈圆形或椭圆形,分散性好;②细胞培养发现,5、10μmol/L浓度的As2O3纳米粒可对人肝癌细胞生长产生明显的抑制作用,并诱导癌细胞凋亡,细胞凋亡率明显高于相同浓度的As2O3溶液组。结论:通过溶胶凝胶法可将As2O3制备成纳米粒子,体外细胞实验证实其抗肝癌细胞的效应强于传统的As2O3溶液。

Objective: To study the method of preparation of As_(2)O_(3 )nanoparticles and its antitumor effects on human liver cancer cells. Methods: As_(2)O_(3 )nanoparticles were prepared by sol-gel method. Microscope, TEM, MTT, Flow cytometry assay(FCM) were performed to examine apoptosis induced by As_(2)O_(3) nanoparticles compared with As_(2)O_(3 )solution at various concentrations(1, 2, 5, 10 μmol/L). Results: As_(2)O_(3 )nanoparticles prepared in our study were round or elliptical, well dispersive and about 80nm and 40 nm in diameter. After 48h of treatment with As_(2)O_(3 )nanoparticles, the survival rate of cells was significant lower than that of As_(2)O_(3) solution with the same concentration(P<0.05). Conclusion: Our research demonstrated that by sol-gel method As_(2)O_(3) could be prepared into nanoparticles. The As_(2)O_(3) nanopaticles could produce a stronger cytotoxic effect on tumor cells than the As_(2)O_(3) solution. This study may develop a new application of As_(2)O_(3) for treatment of solid tumors.