肺静脉源性心房纤颤动物模型中L型钙通道α_(1c)蛋白表达的研究

The protein expression of L-type calcium channel α_(1c) subunit in the animal model of atrial fibrillation of pulmonary vein source

目的:研究肺静脉源性心房纤颤(简称房颤)动物模型中L型钙通道α1c蛋白表达。方法:通过建立兔肺静脉源性房颤的动物模型,并按房颤持续时间或起搏时间的不同分设亚组,应用免疫组化方法检测肺静脉源性房颤组、肺静脉起搏组以及正常对照组的兔肺静脉和心房组织L型钙通道α1c蛋白表达,用图像分析系统对组化抗原表达进行半定量分析。结果:α1c蛋白阳性表达在易发生房颤的动物肺静脉和心房组织中,随着房颤持续时间的延长,出现表达下降,与对照组比较差异有显著性意义(P<0.05);而同样刺激条件下,不能诱发房颤的兔肺静脉和心房组织中未出到α1c蛋白表达的变化(P>0.05)。结论:肺静脉和心房组织L型钙通道蛋白表达的改变可能参与了肺静脉源性房颤的发生和维持,该通道功能的改变可能是肺静脉源性房颤的发生机制之一。

Objective:To investigate the protein expression of L-type calcium channel α_(1c) subunit in the animal model of atrial fibrillation of pulmonary vein source.Methods:After the animal models of atrial fibrillation of pulmonary vein source were set up, the rabbits were divided into different subgroups according to the lasting of atrial fibrillation or the time of pacing. The expressions of L-type calcium channel α_(1c) protein in pulmonary vein and atrial were measured by immunohistochemistry in three groups, which were atrial fibrillation of pulmonary vein source, pacing of pulmonary vein and control group respectively. Semi-quantitative analysis of α_(1c) protein expression was performed with pathological image analytic system. Results:Compared with control group, the protein expression of L-type calcium channel α_(1c) decreased significantly in group of atrial fibrillation of pulmonary vein source with the lasting of time. But the group of pacing of pulmonary vein remained stable. Conclusion:The change of protein expression of L-type calcium channel α_(1c) maybe participate in occurrence and maintain of atrial fibrillation of pulmonary vein source. That functional change of channel maybe one of mechanisms of atrial fibrillation of pulmonary vein source.