摘要
应用四甲基偶氮唑盐(MTT)比色分析法检测细胞的代谢活性及增殖反应。实验结果显示,猴头菇多糖对由刀豆球蛋白(ConA)活化的小鼠胸腺细胞(前T细胞)有较强的促进增殖的作用,其OD值为单独使用刀豆球蛋白A的2倍以上;在脾脏淋巴细胞培养中,单独使用猴头菇多糖可以显著地促进细胞的增殖,其作用较对照组明显。对脂多糖(LPS)刺激的B细胞也有协同作用。
Hericium erinaceus is a Chinese herbal medicine. The Apollo oral liquid contains polysaccharide of H.erinaceus(PHE).The effects of PHE on proliferation of mice T and B lymphocytes were studied.Results showed that(1) 3-fold proliferation of thymocytes was demonstrated when PHE were administrated with Con A than Con A alone.Such effect on thymocyte was not observed when PHE was used alone.(2)Proliferation of spleen lymphocytes was also stimulated when PHE were taken together with lipopolysaccharide,which was 50% and 3 times stronger than that of using lipopolysaccharide and PHE alone respectively.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1994年第7期427-428,共2页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
猴头菇
多糖
淋巴细胞
免疫调节
polysaccharides of Hericium erinaceus,T and B lymphocyte,lipopolysaccharide,Concanavalin A