腺样囊性癌神经侵袭动物模型建立的可行性和特异性研究

Establishing an animal model of neural invasion in adenoid cystic carcinoma:feasibility and specificity

目的:建立腺样囊性癌(ACC)神经侵袭的动物模型。方法:将5×105人涎腺腺样囊性癌ACCM细胞注射入30只裸鼠一侧眶下区皮下,分别于7、11、14、21、28d、动物自行死亡时或56d采集标本,HE染色观察肿瘤能否侵袭眶下神经并测量神经侵袭的长度。另取16只裸鼠,随机分为2组,分别将ACCM和人舌癌Tca8113细胞注射入裸鼠眶下点皮下,14d后HE染色观察肿瘤生长和眶下管内神经侵袭的情况,比较两组神经侵袭的发生率。结果:30只裸鼠模型中23只出现了神经侵袭,7、11、14、21、28d和自行死亡组神经侵袭长度分别为0.09、(0.27±0.02)、(0.38±0.05)、(0.68±0.18)、(1.07±0.36)和(2.10±0.77)mm。另16只裸鼠中8只ACC-M细胞注射裸鼠神经侵袭发生率为100%,远高于Tca8113细胞注射裸鼠的12.5%(P<0.01)。结论:该模型具备良好的可行性、特异性,是腺样囊性癌神经侵袭的良好研究平台。

Objective:To establish an animal model for perineural invasion of adenoid cystic carcinoma(ACC). Methods:Thirty nude mice were subcutaneously injected with 5×10 5 human salivary adenoid cystic carcinoma line-ACC-M cells into one side of infraorbital area. ACC specimens were obtained on the 7th day, 11th day, 14th day, 21st day, 28th day and 56th day after injection or at dying time. H-E staining was performed to identify whether infraorbital nerves were invaded by ACC, and the length of the invaded(infraorbital) nerves was measured. Another 16 nude mice were equally randomized into 2 groups:ACC group and tongue cancer(Tca) group. ACC group was subcutaneously injected with ACC-M cells and Tca group with human tongue cancer line Tca-8113 cells into infraorbital area. Tumor growth and perineural invasion in these 2 groups were observed by H-E staining on the 14th day after injection, and the incidences of perineural invasion in the 2 groups were compared. Results: Perineural invasion was observed in 23 out of 30 nude mice. The length of invaded infraorbital nerves on the 7th day, 11th day, 14th day, 21st day, 28th day and 56th day or at dying time was 0.09, (0.27±0.02), (0.38±0.05), (0.68± 0.18), (1.07±0.36) and (2.10±0.77) mm, respectively. Perineural invasion was observed in all 8 mice in ACC group (100%) and in 1 mice of Tca group(12.5%). A significant difference in the incidence of perineural invasion between ACC group and Tca group was found through Chi-square test(P<0.01). Conclusion: This model has satisfactory feasibility and specificity, which provides a good platform for investigation of perineural invasion of human salivary adenoid cystic carcinoma.