蛋白酪氨酸磷酸酶1B在2型糖尿病初诊患者内脏脂肪组织的表达水平

Expression of tyrosine protein phosphatase 1B in adipose tissues of newly diagnosed diabetic patients

目的:研究蛋白酪氨酸磷酸酶1B(PTP-1B)在2型糖尿病发病中的作用。方法:将34例患者分为对照组、BMI正常的2型糖尿病初诊组(CDM组)和肥胖或超重的2型糖尿病初诊组(ODM组),测定空腹血糖、空腹胰岛素等临床指标,并以Western印迹法测定内脏脂肪组织中PTP-1B的表达水平。结果:ODM、CDM组较对照组已存在明显的胰岛素抵抗,但前两者胰岛素抵抗程度无明显差异,PTP-1B表达水平在上述3组逐渐降低(36.53±14.82,11.57±1.89,3.39±0.94);并且校正年龄和性别后PTP-1B表达水平与BMI呈正相关(r=0.67,P<0.01)。结论:PTP1B表达升高参与了胰岛素抵抗的发生,与2型糖尿病的发病关系密切;但PTP-1B的表达不仅与胰岛素抵抗因素相关,同时还受肥胖等因素的影响。

Objective:To investigate the role of protein tyrosine phosphatase 1B(PTP-1B) in pathogenesis of type 2 diabetes. Methods: Thirty-four patients were divided into 3 groups: type 2 diabetes with normal BMI group(CDM), obese type 2 diabetes group (ODM) and control group. Fasting blood glucose, fasting insulin and body mass were determined. Expression of PTP-1B in adipose tissues were determined by Western blot. Results: Compared with control group, CDM and ODM groups had insulin resistance, though there was no significant difference between CDM and ODM groups. Expression of PTP-1B in control, CDM and ODM groups were 3.39±0.94,11.57±1.89 and 36.53±14.82, respectively. After controling for age and sex, expression of PTP-1B was positively related with BMI(r=0.67, P<0.01). Conclusion: Overexpression of PTP-1B participates in the development of insulin resistance and type 2 diabetes. Not only insulin resistance but also obesity play an important role in expression of PTP-1B.